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Item #:
165669660
Liquid Chromatography Column 227-30106-08 GIS C18
Item #:
165669660
BTN 93624
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GIS C18 Chromatographic columns provide high-resolution separation and are suitable for the analysis of complex samples.
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What Stands Out
High Purity
The GIS C18 column ensures high purity separations, making it ideal for critical applications in pharmaceuticals and environmental analysis, providing reliable results and peace of mind.
Versatile Applications
Designed for a wide range of liquid chromatography applications, the GIS C18 column adapts to diverse sample types, enhancing its usability for laboratories focused on efficiency and flexibility.
Enhanced Durability
Built with robust materials, the GIS C18 column offers exceptional durability and longevity, reducing the need for frequent replacements, thus saving costs and improving laboratory productivity.
Product Details
| Item Weight | 1 lbs (450 grams) |
Who Should Buy?
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Analytical Chemists
Designed for precise analysis, this column is ideal for analytical chemists conducting high-performance liquid chromatography (HPLC) experiments.
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Pharmaceutical Applications
Excellent for drug formulation studies, this column is used in pharmaceutical laboratories for effective compound separation and analysis.
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Research Laboratories
Suitable for research environments, the column can handle complex samples, making it useful for various scientific studies.
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Non-Aqueous Solutions
This column is not suitable for applications involving non-aqueous solvents, as it performs best with aqueous conditions.
Product Description
Liquid Chromatography Column 227-30106-08 GIS C18
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Customer Questions & Answers
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Question:
What is the purpose of the Liquid Chromatography Column 227-30106-08 GIS C18?
Answer: The Liquid Chromatography Column 227-30106-08 GIS C18 is designed for high-performance liquid chromatography (HPLC) applications. Its primary purpose is to separate, identify, and quantify compounds in various mixtures, making it essential for laboratories in pharmaceuticals, biotechnology, and environmental analysis. The C18 stationary phase is known for its hydrophobic properties, which enhance the separation efficiency of non-polar to moderately polar compounds. This makes it particularly useful in analyzing samples like drug formulations, environmental pollutants, and food safety analysis. -
Question:
What are the dimensions and specifications of the GIS C18 column?
Answer: The GIS C18 Liquid Chromatography Column typically features a standard length and diameter, suited for most HPLC instruments. While specific dimensions may vary slightly, they generally fall within the range of 150 mm length and 4.6 mm internal diameter. These specifications help ensure optimal flow rates and separation efficiency. The column packing material is engineered to provide a high surface area for interaction with analytes, contributing to its effective separation capabilities in complex mixtures. -
Question:
How do I maintain the GIS C18 Liquid Chromatography Column for optimal performance?
Answer: To maintain the GIS C18 Liquid Chromatography Column, it's essential to regularly flush the column with an appropriate solvent after use to remove any residual compounds. Additionally, avoiding abrupt changes in mobile phase composition can extend the column’s life. Regularly monitoring back pressure can help in identifying the onset of column degradation. For instance, always store the column under conditions specified by the manufacturer, accessing its full capabilities while minimizing the risk of contamination or misuse. -
Question:
Can I use the GIS C18 column for separating large biomolecules?
Answer: While the GIS C18 column is primarily designed for small to moderate-sized molecules, it can be used for larger biomolecules under specific conditions. C18 columns can provide separation for peptides and small proteins, depending on the configuration of the mobile phase and flow rates. However, for larger biomolecules like antibodies, a size exclusion chromatography approach may be more effective. Always consider the molecular weight and structure of your target compounds to select the appropriate strategy. -
Question:
What types of solvents are compatible with the GIS C18 column?
Answer: The GIS C18 Liquid Chromatography Column is compatible with a wide range of organic solvents such as methanol, acetonitrile, and various buffer solutions. Using appropriate solvents ensures effective elution and separation of analytes. It's important to keep in mind the polarity of your analytes when selecting solvents, as this can impact separation efficiency. For example, a gradient elution method utilizing a mixture of water and organic solvents can enhance the resolution of complex mixtures. -
Question:
What applications is the GIS C18 column best suited for?
Answer: The GIS C18 column is ideal for a variety of applications, including pharmaceutical analysis, environmental testing, and food safety assessments. Its versatility makes it suitable for separating polar and non-polar compounds effectively. For instance, it can be utilized for testing residual solvents in drugs or analyzing pesticide residues in food samples. This adaptability makes it a favored choice among labs needing reliable separation capabilities across different industries. -
Question:
Is the GIS C18 column reusable, and how many runs can I expect?
Answer: Yes, the GIS C18 column is designed for multiple uses, allowing for a significant number of analysis runs before needing replacement. The exact number of runs can vary based on factors such as sample complexity, loading volume, and the conditions of use. Proper maintenance and cleaning between analyses can enhance the lifespan of the column. Users commonly achieve hundreds of runs under optimal conditions, making it a cost-effective choice for routine testing in laboratory applications. -
Question:
How does temperature affect the performance of the GIS C18 column?
Answer: Temperature can significantly influence the retention time and separation efficiency of the GIS C18 column. Higher temperatures may lead to reduced viscosity of the mobile phase, allowing for faster flow rates and shorter run times. However, it can also affect the stability of certain analytes. Users typically find a balance at operational temperatures between 25°C to 60°C to optimize the performance while ensuring the integrity of the samples. Adjusting temperature can be essential in method development for specific applications. -
Question:
What should I do if I experience high back pressure during chromatography with this column?
Answer: High back pressure during chromatography with the GIS C18 column can indicate potential issues such as clogging, column degradation, or improper solvent composition. First, check for particulate buildup by ensuring filters are clean and solvents are clear of contaminants. If the problem persists, consider equilibrating the column with a suitable solvent and re-evaluating your method to ensure proper flow rates are maintained. This may help in diagnosing whether the issue is due to the column or the HPLC system itself. -
Question:
Where can I buy Liquid Chromatography Column 227-30106-08 GIS C18 in Bhutan?
Answer: You can purchase the Liquid Chromatography Column 227-30106-08 GIS C18 through Ubuy in Bhutan. Ubuy is a reliable e-commerce platform that specializes in laboratory supplies and scientific equipment, providing a convenient shopping experience for researchers and technicians. They offer a wide selection of chromatography columns and accessories, making it a go-to destination for obtaining essential laboratory tools.
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BTN 93624
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Features & Benefits
- High-resolution separation for complex sample analysis.
- Versatile for pharmaceutical and various analytical applications.
- Manufactured for long-term repeatability and reliability.
- Supports different separation modes: reversed-phase, normal-phase, affinity chromatography.
- Designed for high flow rates for efficient throughput.
- Ideal for rapid separations and comprehensive analytical needs.
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